Scientist, mRNA Downstream Purification

About NewLimit

NewLimit is a biotechnology company working to radically extend human healthspan. We’re developing medicines to treat age-related diseases by reprogramming the epigenome, a new therapeutic mechanism to restore regenerative potential in aged and diseased cells. We leverage functional genomics, pooled perturbation screening, and machine learning models to unravel the biology of epigenetic aging and disease using experiments of unprecedented scale.

Position

We are seeking a Scientist to lead downstream process development for the mRNA Engineering team. In this role, you will design, optimize, and scale purification processes for mRNA drug substance.

This is a hands-on, lab-based role that spans program process development and platform engineering. It is ideal for a scientist who enjoys solving purification challenges and scaling processes in a dynamic, fast-paced environment.

What you'll do

As a member of our team, you will:

Develop downstream purification processes

Design, execute, and analyze process development studies for mRNA purification

Act as the downstream lead for CMC program teams

Help design and implement automated downstream workflows for high-throughput purification of research-grade mRNA

Support execution of research-grade mRNA production runs for discovery and preclinical studies, as needed

Support process scale-up from bench to pilot scale, as needed

Evaluate emerging purification technologies and chromatography resins

Support tech transfer and CDMO manufacturing

Perform tech transfer of downstream processes to CDMOs

Act as person in plant during CDMO manufacturing runs

Support process troubleshooting and deviation investigations at CDMOs

Documentation and collaboration

Maintain organized experimental records in Electronic Lab Notebooks

Author development SOPs and technical development reports

Present experiment results at team meetings

Requirements

Ph.D. in analytical chemistry, biochemistry, molecular biology, chemical engineering, or a related field with 0–3 years of relevant industry experience, or an M.S. with 5+ years or B.S. with 7+ years of relevant industry experience

Hands-on experience developing downstream purification processes for nucleic acids or other biologics

Hands-on experience with tangential flow filtration (TFF)

Experience operating ÄKTA chromatography systems

Ability to independently design, execute, and troubleshoot experiments

Ability to analyze and present complex data sets

Strong technical writing skills

Excellent communication and organizational skills

Nice to have

Experience with traditional nucleic acid purification methods (e.g., desalting, precipitation, size-exclusion)

Experience with plasmid DNA purification

Experience with Design of Experiments (DoE) and statistical data analysis

Experience implementing automated or high-throughput purification workflows

Experience with process transfer to CDMOs

General understanding of cGMP and phase-appropriate process development

Familiarity with mRNA analytics such as HPLC, CGE, and dsRNA ELISA

Benefits

NewLimit offers generous benefits to ensure our team can focus on our mission:

Health, dental, and vision insurance

401(k) with company match

Paid parental leave

Industry-leading paid time off (PTO) — 20 days/year for all employees + holidays

Meals and snacks on-site

Salary Range: $120,000-155,000*. Compensation for the role will depend on a number of factors, including a candidate’s qualifications, skills, competencies, and experience. NewLimit offers a competitive benefits package, which includes a 401k, healthcare coverage, and 20 days of PTO. Learn more at: https://www.newlimit.com/careers

*This compensation and benefits information is based on Company's good faith estimate as of the date of publication and may be modified in the future.